s * 25 Treatment: Same treatment oti same dates as case (i). After three months daily observation with negative results, the mule was branded with a “T“ and sent to work in the Hamla lines in Khartoum, This mule rapidly gained condition after the treatment and on the 20th October, 1923, 16 months after the treatment, the animal was in good condition. This animal has been in regular work.

Case (iii).

Grey mule. Cast for lameness. Good condition on 13-6-22. It was artificially infected with a strain of Trypanosoma pecaudi (strain No. 145) from a natural case in a mule from the Bahr-el* Ghazal. Five days after infection Trypanosomes appeared in the blood and continued to increase in numbers.

The animal rapidly lost condition and had intermittent fever.

The table shews date of injection, amount, and when the Trypanosome reappeared in the blood .

Date. Injection Date of re-appearance of Trypanosome.

21-6-22 3 grammes of Picric Acid and (3 grammes in alcohol & 4 gramme 29-6-22 in water) T 50 c.c. of Tart? u Emetic sol. 2%. 9-8-22 12-8-22 A course of above dose i.e. L 4 15-8-22 doses, one every third day.

18-8“22 28-8-22 The mule continued to lose condition and had to be destroyed.

Case (iv).

Sudan country-bred horse, cast for lameness, in good condition.

On 1st October, 1922 artificially infected with Trypanosome pecaudi (strain 145). Trypanosomes appeared in the blood on 5th October, 1922. They rapidly increased in numbers and the horse lost condition.

On the 14th, 16th, 18th and 20th October, the following intravenous injections were given : — 3 4 grammes of picric Acid (3 grammes alcoholic and I gramme aqueous) and 50 c. c. of 2% solution of Tartar Emetic.

That is, a course of 4 doses, one every other day.

On the 28th October, 7 days after the last injection, Trypanoso¬ mes reappeared in the blood.

A course of Atoxyl and Picric Acid was then tried on this case,