— 79 — Lubia hellu.

Weight when Dry. Nitrogen. Total N.

Air-dry tops ... 57 gram.

Air-dry roots, with three nodules ... ... 18 /o 3-3 1-08 1-886 •194 Lubia saidi.

Air-dry tops, including 47 pods 430 gram.

Air-dry roots (no nodules) 22 „ 1-8 0-56 7-95 0-123 If may be of interest to add an analysis of the beans which was made at the Imperial Institute (vide Bulletin of the Imperial Institute, Vol. XI., No. 2.).

Moisture Crude Proteins Consisting of-True Proteins .. Other Nitrogenous Substances Fat Starch ...

Kbre ...

Ash Lubia afin.

7-4 23-53 22-7 !2-7 I 0-83 ) 1-05 56-28 7-95 3-79 Lubia hellu.

7-95 20-61 18-53\ 2-08/ 1-63 64-07 2-70 2-98 A glance at these figures will show that where practicable the greatest yield of nitrogen will be obtained by ploughing in the whole plant. Lubia afin has proved the most satisfactory crop on account of the luxuriance of its growth.

The formation of nodules varies greatly with the texture of the soil, light open soils being more favourable than heavy clays. In fact, a prolonged search among plants growing in the latter may not disclose a single nodule.

Inoculation of the seeds with artificial cultures has not resulted in any appreciable success. The nodules are so numerous naturally that where no formation occurs the fault obviously lies with the soil. Inoculation of a plot in which soy beans were growing, with soil from the country of origin was, however, distinctly successful.

It has been found possible to isolate the organisms directly from the soil by means of a synthetic medium containing mannite. The individual organisms of a number of leguminous plants have thus been isolated, but a detailed description of their morphology must form the subject of another paper.