# **Directives** **1. Order to Cease Function of Vital Organs** * *Cessation of Heart Function:* * An injection (30 ml) of 30% potassium chloride (KCl) must be administered into the umbilical vein. * *Cessation of Lung Function:* * An injection (10 ml) of local anesthetic (such as 2% Lidocaine) must be administered directly into the lung tissue.

**2. Additional Procedures (Optional)** * *Brain Removal:* * Brain removal can be performed after the causes of brain death have been confirmed. * *Specimen Collection:* * Tissue samples can be collected for research or educational purposes after obtaining permission from the guardian.

**3. Important Observations** * All procedures must be carried out by a qualified physician. * All procedures performed, their dates, and times must be recorded. * Ensure the presence of two witnesses trained in death procedures.

**4. Death Certificate** * The physician must issue an official death certificate in accordance with legal procedures.

**Note:** These directives are intended for medical use in specific cases and in accordance with applicable regulations.